Dados do Trabalho

Título

OVERWEIGHT AND OBESITY SIGNIFICANTLY INCREASE THE INCIDENCE OF OOCYTE SERC

Objetivos

Metabolic disorders due to obesity alter oocyte's microenvironment and impact oocyte quality. The study purpose was to correlate body mass index (BMI) with the incidence of smooth endoplasmic reticulum cluster (SERC) in oocytes and its consequences on laboratory outcomes.

Método

This is a single-center retrospective study including 80 IVF cycles from 2019 to 2022. Patients were divided into BMI categories groups, according to World Health Organization: underweight (≤18.5kg/m2), healthy (18.5-24.9kg/m2), overweight (25.0 and 29.9kg/m2) and obese (≥30.0kg/m2), 20 each group. Patients and cycle characteristics such as age, AMH, FSH and LH dose, and days of medication were compared among the groups. The incidence of oocyte SERC was blindly evaluated by two senior’s embryologists, through oocyte denuded pictures before ICSI procedure. Additionally, laboratory outcomes such as recovery, maturation, total and top-quality blastocysts, and euploidy rates were compared between groups. Statistical analyses were performed using GraphPad Prism8. One-way ANOVA test was used to compare BMI groups with a post hoc test (Fisher’s) when necessary. Chi-squared test was used to compare proportions, p-value ≤0.05 was considered statistically significant.

Resultados

Obese group demonstrated an increased oocyte SERC incidence when compared with underweight (p=00027) and healthy (p=0.0016) groups. Underweight group had an oocyte recovery rate higher than healthy and overweight groups (p=0.0058 and p=0.0071 respectively). Maturation rate of obese group was higher than underweight (p=0.0117) and overweight (p=0.0330) groups.

Conclusões

These findings support the concept that obesity can contribute to oocyte metabolic disorders and impact oocyte morphology and quality. Further investigation are important to reinforce the results.