Dados do Trabalho

Title

CAN THE USE OF SPERM SELECTION TECHNIQUES INCREASE THE EUPLOIDY RATE?

Objective

To determine if the use of sperm selection techniques, compared to conventional morphological selection before intracytoplasmic sperm microinjection (ICSI) in a group of fertility patients, generates a significant difference in the rate of embryonic euploidy.

Methods

The euploidy results of 181 cycles with donated eggs were analyzed. These procedures were performed as part of the egg donation program of the Germinar Fertility Center (Lima, Peru). The study included 3 groups: 69 cycles with standard morphological selection (ICSI), 56 cycles with selection by physiological ICSI (PICSI) and 56 cycles with selection by annexin columns (CA), for which ejaculated semen samples were used where the initial sample count was greater than 1 million motile sperm. The statistical analysis was by means of a Kruskal Wallis test to compare the proportions of the fertilization, blastulation and euploidy variables with respect to each ICSI, PICSI and CA group.

Results

When comparing the number of fertilized eggs (n=608, n=480, n=522), blastocysts (n=332, n=269, n=285) and euploid embryos (n=175, n=138, n =152) for each of the groups, no differences were observed in fertilization rates (ICSI, 81.6%; PICSI, 80.3%; CA, 81.8%, p=0.54) and blastulation (ICSI, 54.6%; PICSI, 56.0%; CA, 54.6%, p=0.38) among the three groups, an increase in the euploidy rate (72.25%) was observed in the PICSI group compared to CA (66.38%) or conventional ICSI (67.05%), however, it was not statistically significant (p=0.29).

Conclusion

The use of sperm selection techniques such as PICSI or CA on standard morphology selection does not show differences in the fertilization or blastulation rate and does not generate an increase in the embryonic euploidy rate.

Keywords

euploidy, sperm selection, ICSI.