Dados do Trabalho

Title

Definition of a protocol of seminal cryopreservation for Rio de Janeiros’s Sperm Bank.

Objective

The aim of this study is to compare the crioprotetors Spermfreeze™, Ingá Spermfreezing™ and Spermfreeze SSP™ and define a standard operating procedure (SOP) for sperm freezing at Rio de Janeiro’s Sperm Bank

Methods

Samples of patients of Rio de Janeiro Sperm Bank were used. The patients signed a Free and Informed Consent Form, which informs the possibility of using their data in scientific studies. Patients with azoospermia and severe oligospermia were excluded from the study. Before the freezing, the parameters motility, morphology and vitality of the samples were analyzed. The freezing was carried out according to the protocol of each kit and the samples were frozen for a period of one month. After one month, they went through the thawing process to analyze the rates of motility, vitality and sperm morphology.

Results

After the analysis before and after freezing the sample of 13 volunteers, the mean of recovery of sperm motility was 59,21,1±5,027 using Spermfreeze™, 61,15±4,562 using Ingá Spermfreezing and 61,86±6,134 using Spermfreeze SSP™; the mean of recovery of sperm morphology was 91,59±3,299 using Spermfreeze, 86,01±5,536 using Ingá Spermfreezing and 91.20±4,452 using Spermfreeze SSP™, the mean of recovery of sperm vitality was 60,06 ± 4.940 using Spermfreeze, 58.88±6,512 using Ingá Spermfreezing and 56,16±7.275 using Spermfreeze SSP™. No significant difference was found between cryoprotectants in the parameters of survival, motility and sperm morphology.

Conclusion

Aiming the reduction of the use of components of animal origin in the laboratory at Rio de Janeiro’s Sperm Bank and considering the cost-effectiveness of each cryopreservation medium, Fertipro® Spermfreeze™ medium was chosen as the SOP of choice for seminal cryopreservation at Rio de Janeiro’s Sperm Bank.

Keywords

sperm, sperm freezing, male fertility