Dados do Trabalho

Title

Correlation between the time of disappearance of pronuclei and blastulation rate

Objective

The objective of this study was to correlate the blastulation rate of embryos cultured in a Timelapse incubator, with the time in which the pronuclei disappeared.

Methods

After verification of fertilization, patients whose zygotes had two pronuclei were grouped according to the time of disappearance of the pronuclei. 303 zygotes whose pronuclei disappeared before 22 hours after intracytoplasmic sperm injection (ICSI) and 170 zygotes whose pronuclei disappeared after 22 hours of ICSI.

Results

It was observed that the embryos with the best morphokinetic Evolution and higher rate of blatulation were those in which the pronuclei disappeared less than 22 hours after ICSI (78,2%) when compared to zygotes whose pronuclei disappeared after 22 hours after ICSI (48%).

Conclusion

: The time-lapse technology is very useful, as it allows the monitoring of events that would not be possible to be evaluated in conventional cultivation. In the present study, it was evidenced that the time of disappearance of the pronuclei seems to be a predictive value for blastulation rate.

Keywords

timelapse, blastulation, Pronuclei, in vitro fertilization